Application

For use as a Pretreatment Reagent in Immunohistochemical staining procedures. Use Proteinase K to retrieve Epitopes in formalin-fixed, paraffin-embedded tissue sections mounted on glass microscope slides. This procedure is performed after deparaffinization and prior to IHC.



Warm to room temperature before use. Digestion time and dilution should be determined by user as concentration is dependent on the type of antibody, antigen, and fixation method used. Recommended starting dilution range: Neat to 1:30.



For best results use this reagent in conjunction with other IHC Select™ detection reagents. A complete list of reagents is available at www.chemicon.com.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

15 mL in 200 μg/mL

200 µg/mL

Physical form

Liquid Proteinase K containing 15mM sodium azide as a preservative. Discard if it becomes cloudy or contaminated.

Storage and Stability

When stored at 2-8°C, the Proteinase K is stable up to the expiration date printed on the label. Do not freeze or expose to elevated temperatures.