Components

Bovine Fetuin Control, Denaturation Solution, Endo-A-N-Acetylgalactosaminidase, β1,4-Galactosidase, Glucosaminidase, N-Glycosidase F, Neuraminidase, Reaction Buffer, TRITON^™ X-100 Detergent, and a user protocol.

General description

Kit contains all enzymes and reagents needed to remove all N-linked, all simple O-linked, and virtually all-complex O-linked oligosaccharides from glycoproteins in a single reaction at neutral pH. Suitable for use with native or denaturation protocols, with no degradation of protein. This kit has been assembled to contain sequencing grade enzymes without azide, BSA, or glycerol. Each kit can be used to deglycosylate up to 2 mg glycoprotein.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Chiba, A., et al. 1997. J. Biol. Chem.272, 2156.Jiang, M.S., and Hart, G.W. 1997. J. Biol. Chem.272, 2421.Wilkins, P.P., et al. 1996. J. Biol. Chem.271, 18732.Altmann, F., et al. 1995. Glycoconj. J.12, 84.Szkudlinski, M.W., et al. 1995.Endocrinology136, 3325.Pahlsson, P., et al. 1994. Glycoconj. J.11, 43.Saito, S., et al. 1994. J. Biol. Chem.269, 5644.Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol.230, 44.Iwase, H., Hotta, K. 1993. Methods. Mol. Biol.14, 151.Stults, N.L., and Cummings, R.D. 1993. Glycobiology3, 589.Hard, K., et al. 1992. Eur. J. Biochem.205, 785.Kuraya, N., and Hase, S. 1992. J. Biochem. (Tokyo)112, 122.Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem.266, 1646.Fukuda, M., et al. 1987. J. Biol. Chem.262, 11952.Sojar, H.T., and Bahl, O.P. 1987. Arch. Biochem. Biophys.259, 52.Taga, E.M., et al. 1984. Biochemistry23, 815.Kobata, A. 1979. Anal. Biochem.100, 1.Uchida, Y., et al. 1979. J. Biochem (Tokyo)86, 1573.Glasgow, L.R., et al. 1977. J. Biol. Chem.252, 8615.Spiro, R.G., and Bhoyroo, V.D. 1974. J. Biol. Chem.249, 5704.

Packaging

1 kit in Fibre case

Specifications

Assay Time: 3 h-5 days

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)