Application

Iodoacetamide may be used as an alkylating reagent for cysteine residues in peptide sequencing and peptide/protein analytical analysis. It may be used in 2-D protein electrophoresis to reduce streaking and improve resolution by eliminating artifacts that could result from disulfide formation during electrophoresis. By virtue of its reaction with cysteine, iodoacetamide is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibition of ribonuclease. Iodoacetamide may be used in disulfide stress research.

Biochem/physiol Actions

Iodoacetamide acts as an alkylating reagent for cysteine residues in peptide sequencing. It is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibiting ribonuclease.

Legal Information

Technology developed in partnership with Proteome Systems^™

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Packaging

Packaged in sealed ampules

10 vials in glass insert