Application

Antibody suitable for immunoblotting. Working dilution 1:2000Also suitable for dot blot assays and ELISA

General description

The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Immunogen

recombinant polyHistidine tagged fusion protein.

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Reconstitution

The antibody conjugate should be reconstituted with 0.5 ml deionized water.

Specificity

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.