Application

TM[="CelLytic"] B Cell Lysis Reagent has been used for lysing E.coli 5-helix bundle protein. It is also suitable for lysing hepatitis B X-interacting protein (HBXIP) and E. coli TG1 cells.

A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.

Features and Benefits

Higher protein extraction efficiency than traditional methods such as sonication and lysozyme Scalable for 1 to 25 grams of bacterial cell paste No interference with downstream applications such as affinity chromatography, IP, and Western blotting Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents

Legal Information

Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Other Notes

10X concentrate of detergent blend contains no buffering components, giving the researcher flexibility to integrate the lysis reagent into their desired system. Also amenable to automation protocols for recombinant protein extraction and purification.

Packaging

10, 50, 100 mL in poly bottle