Application

Immobilon^®-FL membrane has been used in western blotting. It is also suitable for use in standard chemiluminescent or chromogenic detection.

Features and Benefits

Immobilon^®-FL Membrane: The first transfer membrane designed for fluorescence applications Extremely low background improves the sensitivity of all fluorescence detection protocols Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths compatible with standard blocking agents and buffers Ideal for multiplexing and chemifluorescence detections

General description

The Immobilon^®-FL transfer membrane is hydrophobic polyvinylidene fluoride (PVDF) microporous membrane used for the transfer of proteins from a variety of gel matrices. This transfer membrane offers a uniformly controlled pore structure with a high binding capacity for biomolecules. The membrane displays very low autofluorescence across a wide range of excitation/emission wavelengths. The Immobilon^®-FL membrane has a nominal pore size of 0.45 µm and offers optimal blotting for proteins with molecular weights greater than 20,000 Daltons.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany