Application

Immobilon^® Signal Enhancer is designed for use in Western blotting applications when low signal intensity is observed. It combines signal amplification and blocking in one ready-to-use reagent. Enhancement of the antibody-antigen reaction can produce a 2 to 10-fold increase in signal intensity over traditionally used reagents. The reagent is formulated for use in both membrane blocking and antibody dilution steps and can be used for traditional immunodetection or with the SNAP i.d.™ Protein Detection System. Immobilon™ Signal Enhancer is compatible with PVDF as well as nitrocellulose blotting membranes and can be used with chromogenic, chemiluminescent, and fluorescent detection methods.

General description

Immobilon^® Signal Enhancer combines signal amplification and blocking in one ready-to-use reagent.
Signal Enhancing: Amplify low signal intensity Western blots without amplifying the noise
Save Primary Antibody: When the signal is strong, use Immobilon™ Signal Enhancer to reduce the amount of valuable primary antibody required

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany