Application

Bradford Reagent has been used to determine total protein concentration.

Features and Benefits

The reagent is ready to use. No mixing or dilution required. Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm. Reducing sugars and reducing substances along with thiols do not interfere with this reagent. Reagent is suitable for micro (1-10 µg/ml) and standard (50-1400 µg/ml) assays. Can be used in microwell plate assays. Inexpensive assay.

General description

Bradford assay is addition of coomassie brilliant blue G-250 to protein solution. The coomassie blue dye associates with basic and aromatic amino acids, thereby causing shift in absorbance during protein determination.