Application

Sepharose^® 4B has been used:to conjugate scorpion venom to be used in affinity chromatographyfor clearing of protein extracts prior to immunoprecipitationfor gel purification and desalting of phospholipid vesiclesfor adsorption of immunoglobulin G (IgG)

General description

Sepharose^® 4B is an agarose bead and a polymer of Gal β1,4 [3,6]-anhydro-L-galactose. Sepharose aids in fractionating larger molecules and is devoid of charged saccharides. Presence of 3,6-anhydro-L-galactose resists biological degradation. Hydrogen bonds stabilize the sepharose gel structure. Sepharose is widely used in affinity chromatography by cross linking various ligands on the matrix.

Legal Information

Sepharose is a registered trademark of Cytiva

Packaging

1 L in glass bottle

100, 500 mL in glass bottle