Application

Cyclooxygenase 1 from sheep has been used as positive control protein in western blot analysis of osteoarthritis samples and in prostaglandin synthase activity assay.

Biochem/physiol Actions

COX-1 catalyzes the conversion of arachidonic acid to prostaglandin H₂ (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is involved in the homeostatic role of eicosanoids and constitutively expressed in almost all animal tissues. Has an apparent K_M of 8.3 µM for arachidonic acid.

General description

Cyclooxygenase 1 is a 71 kDa membrane bound protein predominantly present in endoplasmic reticulum. It has three domains, the epidermal growth factor (EGF) like domain, enzymatic and membrane binding domain. Cyclooxygenase 1 mediates prostaglandin synthesis and is modulated by anti inflammatory nonsteroidal drugs.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Physical form

Solution in 80 mM Tris-HCl, pH 8, with 0.1% TWEEN^® 20, 300 µM diethyldithiocarbamate and 10% glycerol.

Unit Definition

One unit consumes one nanomole of oxygen per minute at 37 °C in 0.1 M Tris-HCl buffer, pH 8, containing 100 µM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 µM hematin.