Analysis Note

Tested for the ability to catalyze the 7-deethylation of ethoxyresorufin.

Biochem/physiol Actions

Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous.

Physical form

Solution in 100 mM potassium phosphate buffer, pH 7.4.

Preparation Note

Microsomes containing human CYP1B1 and recombinant human NADPH-P450 reductase.

Unit Definition

One unit will reduce 1 nanomole of cytochrome C per minute at pH 7.4 at 37 deg C.