Amino Acid Sequence

NFor-Met-Leu-Phe

Application

N-Formyl-Met-Leu-Phe has been used for:creating gradients in Zigmond chamber chemotaxis assay performed on neutrophilsuse in neutrophil chemotaix assay anddetermination of the involvement of MAPK-activating protein kinase-2 (MAPKAPK-2) and/or p38, in the signaling pathway of human polymorphonuclear leukocytes (PMNs) stimulated by N-Formyl-Met-Leu-Phe.

Biochem/physiol Actions

Potent inducer of leucocyte chemotaxis and macrophage activator. Receptors that bind formylpeptides are found on phagocytic neutrophils and have recently been identified on cells of the intestinal mucosa.

N-Formyl-Met-Leu-Phe induced chemotaxis in phosphoinositide 3-kinase γ (PI3K)γ^-/- neutrophils, and promotes adhesion, polymerization of F-actin, Fcγ receptor-mediated phagocytosis and intracellular Ca²⁺ release. It acts as an inflammatory agent and activates polymorphonuclear leukocytes (PMNs) without the formation of 5-hydroxyicosatetraenoic acid (5-HETE) and leukotriene B₄ (LTB₄). Its effects on neutrophils and the inhibition of adenylate cyclase are inhibited by pertussis toxin.

Potent chemotactic peptide. Induces a metabolic burst in macrophages accompanied by an increase in respiratory rate, secretion of lysosomal enzymes, and production of superoxide anion.

General description

N-Formyl-Met-Leu-Phe is a chemotactin peptide, which functions on polymorphonuclear leukocytes (PMN), neutrophils etc.

Packaging

5, 10 mg in glass insert

50, 100, 500 mg in glass bottle