FGF2-G3 (145aa) protein is a thermostable engineered form of FGF-2 (bFGF). Qk052 comprises the 145aa form of FGF-2 (Qk025) with the nine amino acid substitutions developed by Dvorak et al. 2018. This increases the functional half-life of the protein from 10 h (wild-type) to >7 days (FGF2-G3 145 aa).

FGF2-G3 is used in B8 media (Kuo et al. 2019) for weekend free, high homogeneity induced pluripotent stem cell culture. This new 145aa thermostable FGF-2 protein allows direct comparison of efficacy in systems using FGF-2 145aa (Qk025) for chemically defined stem cell and organoid culture media, and cultured meat media development.

High purity 16 kDa bioactive FGF2-G3 145aa protein. Animal origin-free (AOF), carrier protein-free and with no His-tag.

• High purity thermostable FGF2-G3 protein comprising 145aa form of FGF-2 (Uniprot: P09038) with nine stabilising amino acid substitutions
• >98%, by SDS-PAGE quantitative densitometry
• 16 kDa
• Expressed in E. coli
• Animal origin-free (AOF) and carrier protein-free
• Manufactured in our Cambridge, UK laboratories
• Lyophilised from Tris/NaCl/CyS/mannitol
• Resuspend in water at >100µg/mL, prepare single use aliquots, add carrier protein if desired, store frozen at -20°C (short-term) or -80°C (long-term)