Recombinant FGF2-G3 (FGF2-STAB®) protein is a thermostable engineered form of FGF-2 (bFGF). Qk053 is the 154 aa mature domain of FGF-2 (Qk027) with nine amino acid substitutions to enhance stability without impacting bioactivity developed by Dvorak et al. 2018. This increases the functional half-life of the protein from 10 h (wild-type) to >7 days (FGF2-G3).
Recombinant FGF2-G3 is used in B8 media (Kuo et al. 2019) for weekend free, high homogeneity induced pluripotent stem cell culture. FGF2-G3 also has applications in chemically defined stem cell and organoid culture media, and cultured meat media development.
High purity 17 kDa bioactive FGF2-G3 protein. Animal origin-free (AOF), carrier protein-free and with no His-tag.
- High purity thermostable recombinant FGF2-G3 protein comprising 154aa form of FGF-2 (Uniprot: P09038) with nine stabilising amino acid substitutions
- >98%, by SDS-PAGE quantitative densitometry
- 17 kDa
- Expressed in E. coli
- Animal origin-free (AOF) and carrier protein-free
- Manufactured in our Cambridge, UK laboratories
- Lyophilised from Tris/NaCl/CyS/mannitol
- Resuspend in water at >100µg/mL, prepare single use aliquots, add carrier protein if desired, store frozen at -20°C (short-term) or -80°C (long-term)