Dickkopf-related protein 1 (DKK-1) is a key player in the Wnt signalling pathway and regulates embryonic development, tissue balance, and diseases, including cancer. DKK-1 is a member of the DKK protein family and acts as a potent Wnt pathway antagonist.

In cell culture, DKK-1 is pivotal for controlling cell fate, self-renewal, and differentiation by inhibiting the canonical Wnt/β-catenin pathway through LRP5/6 co-receptors. DKK-1 has been widely used in various applications including guiding stem cell differentiation, particularly in neural and osteogenic pathways, and mimicking dysregulated Wnt signalling seen in cancers. Its inhibitory role is also crucial for anterior development.

Human DKK-1 has a molecular weight of 25.7 kDa, animal origin-free, carrier-free and tag-free to ensure its purity with exceptional lot-to-lot consistency. DKK-1 is suitable for the culture of reproducible and high-quality stem cells and other relevant cells.

• High purity human DKK-1 protein (Uniprot number: O94907)
• >98%, by SDS-PAGE quantitative densitometry
• Source: Expressed in E. coli
• 25.7 kDa, monomer
• Animal origin-free (AOF) and carrier protein-free
• Manufactured in Cambridge, UK
• Lyophilised from acetonitrile/TFA/mannitol
• Resuspend in water at >100µg/mL, prepare single-use aliquots, add carrier protein if desired and store frozen at -20°C or -80°C