Analysis Note

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Application

Acetylcholinesterase human has been used: as a standard protein to measure protein levels acetylcholinesterase AChE-R and AChE-Sto stimulate human fibroblaststo study its in vitro catalytic activity and to determine the effects of metals, H₂O₂ and OH radicals on the activity

Biochem/physiol Actions

Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H₂O to choline + acetic acid.

Acetylcholinesterase(AChE) isregarded as abiomarker inneurotoxicity. It is a modulator ofnitric oxidesignal transduction pathway andmarker of membrane integrityand aging.AChE, hydrolyzes choline esters. It terminates the impulse transmission at cholinergic synapses. AChE does this by rapid hydrolysis of the neurotransmitter acetylcholine (ACh) to acetate and choline. AChE inhibitors prevent the cholinesterase enzyme from breaking down ACh and increases the level and duration of the neurotransmitter action.

General description

Acetylcholinesterase(AChE) is a serine hydrolase, whichbelongs to the carboxyl esterase family of enzymes. AChE is localised at neuromuscular junctions and cholinergic brain synapses.

Packaging

0.1 mg in serum bottle

Physical form

This product is supplied as a lyophilized powder. Lyophilized from 0.22 µm filtered solution in 50mM phosphate buffer pH8.

Unit Definition

One unit will hydrolyze 1.0 µmole of acetylthiocholine to thiocholine and acetate per minute at pH 8.0 at 37 °C.