Application

Coelenterazine, native was used:-For Aequorin assaysFor measuring the intracellular calcium concentration in response to ionomycin or PMA using aequorin in COS-7 cellsIn Bioluminescence imaging for analysing the presence of virus-dependent luciferase activity

Biochem/physiol Actions

Luminophore of the aequorin complex which is oxidized by oxygen to illuminate at 465 nm when Ca²⁺ binds to the complex; used to measure Ca²⁺ concentration in cells with high sensitivity and large dynamic range.

Caution

Protect from exposure to light

General description

Coelenterazine is a luciferin molecule, which is utilized by several luciferases, such as Renilla,Oplophorus andPeriphylla.