Analysis Note

Protein determined by Lowry.

Application

C Reactive Protein from human fluids has been used:to investigate the neutrophil extracellular traps (NETs)-dependent generation of monomeric CRP (mCRP) from pentameric CRP (pCRP)to evaluate its effects on areas at risk (AAR) of myocardium with ischemia-reperfusion injuryto measure intracellular levels of reactive oxygen species (ROS)

Biochem/physiol Actions

C Reactive Protein (CRP) plays a critical role in apoptosis, phagocytosis, nitric oxide (NO) release and the secretion of cytokines, such as interleukin-6 and tumor necrosis factor-α. CRP acts as a marker for cardiovascular diseases, rheumatoid arthritis and cancer.C reactive protein (CRP) is an acute phase protein. Serum levels in patients with atherosclerosis is predictive of increased risk of myocardial infarction (MI) and stroke. The cytokine IL-6 is thought to be the key mediator in hepatocyte secretion of acute phase proteins including CRP. CRP mediates innate immunity by binding to microbial polysaccharides and to ligands exposed on damaged cells. The binding activates the classical complement pathway (C1, C4, C2, C3 but not C5-9). Opsonization of the substrates leads to their uptake by phagocytic cells and limits the inflammatory response.

General description

C Reactive Protein (CRP) isa homopentameric protein, which belongs to the pentraxin family. It is produced in liver hepatocytes, smooth muscle cells, macrophages and adipocytes. CRP gene is located on human chromosome 1q23.2.

Other Notes

CRP is isolated from human fluids (Ascitic/Pleural).

Packaging

Package size based on protein content.

Physical form

Solution in 0.02 M Tris, 0.28 M sodium chloride, 0.005 M calcium chloride, pH 7.8 - 8.2, containing 0.1% sodium azide.