Analysis Note

Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria.

Biochem/physiol Actions

Caspase-8-cleaved BID relocates from the cytosol to the outer mitochondrial membrane where its interaction with Bak alters mitochondrial membrane permeability.

General description

Mouse BID cleaved with caspase-8 generates the amino-terminal fragment (amino acids 1-59, 7 kDa) and the carboxy-terminal fragment (amino acids 60-195, 15 kDa). On size exclusion chromatography cleaved BID elutes at 27 kDa indicating that the fragments remain associated.

Physical form

0.2 µm filtered solution in 25 mM HEPES, pH 7.5, and 0.1 M KCl.