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ISOLATE II RNA Mini Kit is specially designed for the fast and efficient isolation of extremely pure total RNA from a variety of sources. The kit is compatible with animal tissues, cultured cells, bacterial cells, yeast and biological fluids (cell-free). Biological samples which are sometimes difficult to process will yield high-quality RNA i.e. mouse tissue (liver, brain), various tumour cell lines, Streptococcus and Actinobacillus pleuropneumoniae. The sample is lysed with an optimized lysis buffer containing chaotropic ions, which simultaneously inactivates RNases, preventing degradation of the released RNA. The lysate is then applied to a spin column to selectively remove genomic DNA (RNase-free DNase I is supplied with the kit). There is no need to perform a separate DNase digestion step. The RNA is bound to the silica membrane, washed and pure, high quality RNA is eluted . The online product manual has protocols for purifying total RNA from cultured cells, tissues, yeast, bacteria, biological liquids, paraffin embedded tissue and RNAlater® treated samples, as well as DNase I treatment of purified RNA and clean-up of RNA from reaction mixtures. The isolated RNA shows excellent performance in downstream applications such as reverse transcription, one-step real-time PCR , Northern blot analysis, microarrays and RNA protection assays.

Features

• Rapid protocol: 30 min/6 preps
• High purity RNA (A260/A280 ratio 1.9 - 2.1)
• RNA integrity number (RIN) >9
• Extra filters (shredders) included to enhance sample homogenization
• DNase I included for complete removal of genomic DNA

Applications

• Isolation of RNA from: Animal tissue, Eukaryotic cells, Bacterial cells, Yeast, Biological fluids (cell-free)
• Isolated RNA is ready for downstream applications such as reverse transcription and one-step real-time PCR

Components: ISOLATE II Filters, ISOLATE II RNA Mini Columns, Collection Tubes, Lysis Buffer, RLYWash Buffer, RW1Wash Buffer, RW2 Membrane Desalting Buffer, MEM Reaction Buffer for DNase I, RDN DNase I, RNase-free (lyophilized) RNase-free water

Storage Conditions: ISOLATE II RNA Mini Kit contents should be stored dry at room temperature. Under these conditions, the kit is stable for 12 months.