ISOLATE II RNA Plant Kit is specially designed for the fast and efficient isolation of extremely pure total RNA from a variety of plant tissues, including leaves, bark, roots and fruits. Up to 100mg starting material can be processed per spin column. The protocol is easy to follow on a step-by-step basis. Two highly optimized lysis buffers, containing guanidinium thiocyanate and guanidinium-HCl are provided to ensure lysis for all plant types. The lysis buffers also inactivate RNases, thus protecting the released RNA. The lysate is applied to a spin column to selectively remove contaminating genomic DNA, eliminating the need to perform a separate DNase I digestion step. The RNA is then bound to a silica membrane. Subsequent wash steps remove the remaining cell debris and pure RNA is eluted in the final step with RNase-free water. The isolated RNA shows excellent performance in downstream applications such as real-time PCR , reverse transcription, Northern blot analysis, microarrays and RNA protection assays.

Features

• Rapid protocol: 30 min/6 preps
• High-purity RNA (A260/A280 ratio: 1.9 - 2.1)
• Excellent RNA recovery and integrity (RIN>9)
• Extra filters (shredders) to enhance sample homogenization included
• DNase I included for complete removal of contaminating genomic DNA

Applications

• Isolation of RNA from: Fresh plant cells and tissue
• Frozen and lyophilized plant tissue
• Filamentous fungi
• Isolated RNA is ready for downstream applications such as real-time PCR and reverse transcription

Components: ISOLATE II Filters, ISOLATE II RNA Plant Columns, Collection Tubes, Lysis Buffer, PA1 Lysis Buffer, PA2 Precipitation Buffer, PL3 Binding Buffer, PB Wash Buffer, PAW1 Wash Buffer, PAW2 (Concentrate) Elution Buffer, PG RNase A (lyophilized)

Conditions: ISOLATE II RNA Plant Kit contents should be stored dry at room temperature. Under these conditions, the kit is stable for 12 months.