Biochem/physiol Actions

The size and monovalency of this product provide advantages over tetrameric streptavidin in applications such as functional cell surface labeling and atomic force spectroscopy. These advantages include: Minimizing distribution or dynamics in the probing of biotinylated targets Reducing risk of aggregation, precipitation, and cross-linking in pre-incubation steps with biotinylated molecules Easier access to desired targetThis product can be used in traditional biotin-binding applications, including Western blotting, immunoprecipitation, immunohistochemistry, and flow cytometry.

SAvPhire Monomeric Streptavidin is a variant of the biotin-binding streptavidin, which naturally exists as a tetramer. Many monomeric streptavidin proteins have rapid dissociation kinetics, which prevent their use in stable labeling applications. However, SAvphire Monomeric Streptavidin has been engineered to have a dissociation rate comparable to that of tetrameric streptavidin.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Physical properties

Sequence contains N-terminal 6×-Histidine-tag and a C-terminal FLAG-tag

Reconstitution

This product is lyophilized from PBS, pH 7.4. Reconstitute the vial contents to a 4 mg/mL solution by adding water as follows: 1 mg vial: add 250 µL of water for a 4 mg/mL solution 5 mg vial: add 1.25 mL of water for a 4 mg/mL solutionThe reconstituted product can be further diluted in water or PBS as needed.

Once reconstituted, the solution can be stored at 2-8 °C for at least 1 month. For long term storage, aliquot and store at -20 °C.