Oz Biosciences GFP Reporter Gene mRNA Modified

GFP mRNA has been designed to produce high expression level of green fluorescent protein. It can be used as control of transfection efficiency. It is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509nm. OZ Biosciences mRNAs are produced by in vitro transcription and stabilised at the 5' end by modified nucleotides capping (Cap1). These mature mRNAs contain a poly(A) tail at the 3' end and are also optimised to yield improved stability and performance.

• MRNA15 does not bear any additional nucleotide modifications
• MRNA11 is modified with 5-methoxyuridine (5moU) to reduce innate immune response
• MRNA22 is modified with N1-methyl-pseudouridine (N1-mψ) to reduce innate immune response
• MRNA15c and #MRNA11c are labelled with Cy5 (Sulfo-Cyanine5) by replacing 15 % of U or moU by UTP-Cy5