OZB self-amplifying saGFP RNA has been constructed based on replicons of positive-sense ((+)-RNA) viruses (Venezuelan equine encephalitis Virus (VEEV)), where the coding sequence of the viral structural proteins is replaced with that of a gene of interest (GOI, here GFP), while retaining the coding sequences of non-structural proteins (NsPs), including the viral RNA-dependent RNA polymerase. Please note that OZB saRNAs encodes also for Puromycin Resistance Cassette, which does not alter the expression of the GOI but can be used for cell selection.

SaGFP RNAs have been designed to produce high expression level of GFP protein with enhanced length of duration. OZB mRNAs are produced by in vitro transcription. mRNAs are stabilised at the 5’ end by modified nucleotides capping (Cap1) and contain a poly(A) tail at the 3’ end. Sequences have been optimised to yield improved stability and performance.

• MRNA70 does not bear any additional nucleotide modifications
• MRNA71 is modified with 5-methyl-Cytidine (m5C) (100% replacement of Cytidine)
• Ready-to-use stabilised saGFP puroR RNA
• Concentration: 1.0mg/mL in 1mM Sodium Citrate, pH 6.4
• mRNA length: 9821nt
• MRNA70 Molecular weight: 3,17 E+06g/mol
• MRNA71 Molecular weight: 3,20 E+06g/mol
• NAs must be stored at -80°C
• We recommend to aliquot the RNA solution for a better storage and avoid freeze/thaw cycles